Mineralogical analysis by electron microscopy and Laser Induced Breakdown Spectroscopy (LIBS) in two patients with sarcoidosis from the MINASARC study (French)

The MINASARC study is a case-control study (20 cases of sarcoidosis and 20 healthy volunteers) designed to evaluate the inorganic « exposome » by a occupational and environmental questionnaire concomitant with mineralogical analysis (AM) on bronchoalveolar lavage ( LBA) in transmission electron microscopy (MET) and biopsies embedded in paraffin by light microscopy (OM) and scanning electron microscopy (SEM). This study showed that 8 cases of sarcoidosis and 5 healthy volunteers had high mineral particle loads, but no significant difference between the two groups except for the steel load (p = 0.029). In addition, the questionnaire showed that the activity of construction and demolition was significantly higher in patients with sarcoidosis (p = 0.018) [1]. We report, in this poster, the results of the analysis in electron microscopy obtained on two of the cases of sarcoidosis, compared to an analysis in LIBS (Laser Induced Breakdown Spectroscopy) [2] performed on biopsies.