This study began in 2013 and compares the results of the dust collection questionnaire and the mineralogical analysis in 20 subjects suffering from sarcoidosis recruited in four pulmonology departments (Avicenne Hospital, Louis Pradel Hospital, Lyon Sud Hospital Center and Hôpital de la Tronche in Grenoble ) and 20 healthy subjects, matched by sex, smoking and age, recruited by the company Dermscan and having a pneumological assessment at the Saint Joseph and Saint Luc Hospital Center. The inclusion of patients ended in the summer of 2015 and the mineralogical analyzes, carried out in Transmission Electron Microscopy at the Faculty of Medicine of Lyon, were completed in September. A meeting to lift the anonymity of the samples analyzed by electron microscopy took place at the Saint Joseph and Saint Luc Hospital on October 8th, 2015. A first article as been accepted by Sarcoidosis, Vasculitis and Lung Diseases.
This is a study promoted by the Paris Public Assistance and in particular the Orphan Disease Center at Trousseau Hospital. The principal clinical investigator is Dr. Nadia Nathan, Mr. Vincent is co-investigator. The Minapath Company was a partner in the study by sending an indoor air counselor to sarcoidosis affected children's home.
The complexity of the osteoarticular pathologies makes that currently there are no really effective treatments, except the installation of an implant (arthroplasty). In 2013, 0.8% of Americans and 0.1% of Europeans had a hip implant. That's a global market of about $ 6.3 billion, up 1.7% a year [avicenne.com]. In addition to this economic issue, there is also a societal stake. For the year 2012, in France, the cost of medical care associated with arthroplasties amounts to about 2 billion euros for Social Security (world spending is 20 times greater). On the other hand, despite many efforts to delay implant replacement and develop new materials to minimize wear, the in vivo duration of joint implants is often very disappointing compared to extrapolations made from ex vivo simulations. Thus, recent studies show, on the one hand, the increase in allergic reactions to the materials used to make these joint implants and, on the other hand, the increase in nano-toxicity reactions related to the presence of ultrafine particles, which significantly reduces the life of the in vivo implant. In this context, the goal of this project is to develop a new bio-tribo-mineralogical expertise protocol for arthroplasty specimens to identify the most toxic particle classes. This will then help clinicians in the detection of pathologies related to fine wear particles and manufacturers to optimize the tolerance of implants by limiting the production of wear particles.This project, in partnership MINAPATH / LaMCoS, is funded by a European Regional Fund (FEDER).
The group of interstitial lung diseases includes over 200 non-infectious conditions, more than 65% being categorized as idiopathic. Those diseases are sometimes associated to an exposure to inorganic dusts, which accumulate in the lungs causing inflammation, granulomatosis, fibrosis and eventually cancer. Those interstitial lung diseases include among others: sarcoidosis, idiopathic pulmonary fibrosis and infiltrative pneumonia associated with connective tissue diseases. Our study aims at measuring the mineral exposome in 300 retrospective cases of interstitial lung diseases, and at evaluating the possible causal relationship between exposome and interstitial lung diseases heretofore diagnosed as idiopathic. The mineral exposome will be measured by: 1) an in depth questionnaire, 2) a mineralogical analysis of paraffin-embedded lung biopsy using Scanning Electron Microscopy coupled with Energy Dispersive X-ray (SEM/EDX), Inductively Coupled Mass Spectrometry (ICP-MS) and Laser Induced Breakdown Spectroscopy (LIBS) techniques and 3) a hypersensitivity test on blood sample by Lymphocyte Proliferation Test (LPT). Causal relationship will be established using Hill’s criteria. The study will evaluate the proportion of cases that can be related to exposure (non-idiopathic disease). The MINAPILD study based on a systematic in depth questionnaire and mineralogical analysis may alter the paradigm of approach to the diagnosis of interstitial lung diseases, with potential impact on indications of bronchoalveolar lavage and lung biopsy.
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 787681
Other research projects
With internal medicine services for the study of questionnaire intake and / or mineralogical analysis for patients with disseminated lupus erythematosus, scleroderma, vasculitis. With rheumatology services for rheumatoid arthritis studies. With pulmonology and thoracic oncology services for studies on the role of nonfibrous mineral particles in triggering thoracic cancers. With general oncology services for studies on the role of mineral particles or fibers in triggering diseases such as lymphoma, ear, nose, throat, kidney, ovarian cancers ...